Rapi-D™ Quantitative Vitamin D Test utilises the principle of Immunochromatography, a unique two-site “Sandwich” immunoassay on a membrane. The test employs a very “Exclusive” pair of anti-25-OH Vitamin D Monoclonal Antibodies; one conjugated with colloidal gold and another one immobilized on the solid phase. This will selectively detect Vitamin D with a high degree of sensitivity and specificity.
As the test sample flows through the membrane assembly within the test device, the coloured anti-25-OH Vitamin D-colloidal gold conjugate complexes with 25-OH Vitamin D from the sample. This complex moves further on the membrane by the capillary action to the test region (T) where it is immobilized by another anti-25-OH Vitamin D coated on the membrane, leading to formation of a pink / purple coloured band, which confirms a positive test results. The intensity of coloured band in the test line region is 25-OH Vitamin D concentration-dependent, higher the concentration of 25-OH Vitamin D in the tested sample, the stronger the coloured band is. A control line is present in the test window to work as procedural control.
Important notes
To be used by trained healthcare professionals. This is an in vitro screening tool to support and enable diagnosis.
Additional Features | Test Kit Includes
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Type of Measurement
Sample size/volume
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Storage
Operating Temperature
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* Established 2001